Ion Exchange Chromatography: YMC Bio Pro
YMC-BioPro ion exchange columns are for the separation of proteins, peptides, and nucleic acids. YMC-BioPro IEC columns are available in QA and SP chemistries and are based on porous (QA and SP columns) and non-porous (QA-F and SP-F columns) hydrophilic polymer beads with low nonspecific adsorption. The porous materials offer excellent binding capacity with exceptionally high efficiency. The non-porous particles offer high efficiency and exceptional resolution.
Specification
| YMC-BioPro QA | YMC-BioPro SP | YMC-BioPro QA-F | YMC-BioPro SP-F | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity (meq/mL-resin) | 0.075-0.100 | 0.070-0.095 | 0.075-0.110 | 0.230-0.290 |
| Dynamic binding capacity (mg/mL-resin) | >110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4 - 60 ℃ | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
POINTS
- Newly developed hydrophilic polymer beads with low nonspecific adsorption
- Effective surface structure designed to interact with biomolecules
- High theoretical plate number and symmetrical peak shape by optimal packing technology
- QA (quaternary ammonium) and SP (sulfopropyl) ion exchangers
- Ideal for analysis and laboratory-scale purification on porous type with high binding capacity and high recovery of biomolecules
- Ultra-fast analysis and high-resolution analysis on non-porous type
APPLICATION DATA
Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns
The separation of MAb is compared on SCX (YMC-BioPro SP-F) and WCX (competitor’s) under the same gradient conditions at pH 5.6.
The YMC-BioPro SP-F column provides higher resolution of MAb in a shorter analysis time than the competitor's column.
Excellent batch-to-batch reproducibility
The YMC-BioPro SP-F column exhibits excellent batch-to-batch reproducibility for MAb analysis, including the resolution of peaks for small charge variants. All the gel batches are inspected by various quality control tests, including HPLC analysis of MAb, and must pass rigorous criteria before release. YMC-BioPro ion exchange columns are the best choice for the quality control of MAb and other biopharmaceuticals.
30 mm-length column for high-throughput analysis with low operating pressure
The separation performance of non-porous and porous columns for proteins is compared. YMC-BioPro non-porous types are effective for high-throughput analysis at a high flow rate because of its high mechanical strength and high column efficiency.
High binding capacity and high recovery of biomolecules
Compared with conventional porous-polymer anion-exchange columns, YMC-BioPro QA has a superior DBC. Furthermore, its recovery is greater than that of conventional columns. The hydrophilic properties of the matrix polymer remarkably reduce the nonspecific adsorption of proteins on YMC-BioPro columns.
Comparisons in dynamic binding capacity (DBC) and recovery for BSA
| Dynamic binding capacity (mg/mL-resin, 10% breakthrough) |
Eluted amount (mg/mL-resin) |
Recovery* (%) |
|
|---|---|---|---|
| YMC-BioPro QA | 126 | 120 | 95 |
| Brand T (porous Q type) | 73 | 58 | 79 |
| Brand G (porous Q type) | 100 | 35 | 35 |
*Recovery: (Eluted amount/Dynamic binding capacity) X 100
Breakthrough curves
High loadability
YMC-BioPro QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot achieve acceptable peak shapes and resolution even in small amount of injection.
Excellent peak shapes even at high sample load
YMC-BioPro
| Packing material | Particle size (µm) |
Pore size | Column size inner diameter x length (mm) |
Product number |
|---|---|---|---|---|
| QA | 5 | Porous | 4.6 X 30 | QAA0S05-0346WP |
| 4.6 X 50 | QAA0S05-0546WP | |||
| 4.6 X 100 | QAA0S05-1046WP | |||
| SP | 5 | Porous | 4.6 X 30 | SPA0S05-0346WP |
| 4.6 X 50 | SPA0S05-0546WP | |||
| 4.6 X 100 | SPA0S05-1046WP | |||
| QA-F | 3 | Non-porous | 4.6 X 30 | QF00S03-0346WP |
| 4.6 X 50 | QF00S03-0546WP | |||
| 4.6 X 100 | QF00S03-1046WP | |||
| 5 | Non-porous | 4.6 X 30 | QF00S05-0346WP | |
| 4.6 X 50 | QF00S05-0546WP | |||
| 4.6 X 100 | QF00S05-1046WP | |||
| SP-F | 3 | Non-porous | 4.6 X 30 | SF00S03-0346WP |
| 4.6 X 50 | SF00S03-0546WP | |||
| 4.6 X 100 | SF00S03-1046WP | |||
| 5 | Non-porous | 4.6 X 30 | SF00S05-0346WP | |
| 4.6 X 50 | SF00S05-0546WP | |||
| 4.6 X 100 | SF00S05-1046WP |