Size Exclusion Chromatography:YMC Pack Diol
YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.
Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.
Specification
Column | Pore size (Å) |
Particle size(µm) | Usable pH range | characteristics |
---|---|---|---|---|
Diol-60 | 60 | 3, 5 | 5.0-7.5 | For molecular weight below 10,000 |
Diol-120 | 120 | For molecular weight 5,000 to 100,000 | ||
Diol-200 | 200 | For molecular weight 10,000 to ca. 500,000 | ||
Diol-300 | 300 | For molecular weight ca. 50,000 to 1,000,000 |
POINT
- Utilizes silica gel base with high resistance to pressure
- Low-cost gel filtration column
- Useful for molecular weight determination of proteins and sugars
- Minimal secondary interactions
APPLICATION DATA
Calibration curves of proteins obtained using columns with different pore sizes
Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
Column | YMC-Pack Diol 300 x 8.0 mmI.D. |
---|---|
Eluent | 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl |
Flow rate | 0.5 mL/min |
Temperature | 25°C |
Detection | UV at 280 nm |
For separation of proteins with molecular weights of 10,000 to several hundred thousands

Column | YMC-Pack Diol-SEC 500 x 8.0 mmI.D. |
---|---|
Eluent | 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl |
Flow rate | 0.7 mL/min |
Temperature | ambient |
Detection | UV at 280 nm |
For separation of peptides with molecular weights of 10,000 or less

Column | YMC-Pack Diol-60 500 x 8.0 mmI.D. |
---|---|
Eluent | 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl /acetonitrile (70/30) |
Flow rate | 0.7 mL/min |
Temperature | ambient |
Detection | UV at 215 nm |

For molecular weight determination of sugars
For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination.
Column | YMC-Pack Diol, 500 x 8.0 mmI.D. |
---|---|
Eluent | water |
Flow rate | 1.0 mL/min |
Temperature | ambient |
Detection | RI |

Analysis Columns(Stainless Columns)
Packing material | Particle size (µm) |
Pore size (Å) |
Column size Inner diameter x length (mm) |
Product Number |
---|---|---|---|---|
Diol-60 | 3 | 60 | 4.6 X 300 | DL06S03-3046WT |
5 | 60 | 4.6 X 300 | DL06S05-3046WT | |
8.0 X 300 | DL06S05-3008WT | |||
8.0 X 500 | DL06S05-5008WT |
|||
Diol-120 | 3 | 60 | 4.6 X 300 | DL12S03-3046WT |
5 | 60 | 4.6 X 300 | DL12S05-3046WT | |
8.0 X 300 | DL12S05-3008WT |
|||
8.0 X 500 | DL12S05-5008WT |
|||
Diol-200 | 3 | 60 | 4.6 X 300 | DL20S03-3046WT |
5 | 60 | 4.6 X 300 | DL20S05-3046WT | |
8.0 X 300 | DL20S05-3008WT |
|||
8.0 X 500 | DL20S05-5008WT |
|||
Diol-300 | 3 | 60 | 4.6 X 300 | DL30S03-3046WT |
5 | 60 | 4.6 X 300 | DL30S05-3046WT | |
8.0 X 300 | DL30S05-3008WT |
|||
8.0 X 500 | DL30S05-5008WT |
Guard Columns(Stainless Columns)
Packing material | Particle size (µm) |
Pore size (Å) |
Column size Inner diameter x length (mm) |
Product Number |
---|---|---|---|---|
Diol-60 | 5 | 60 | 8.0 X 30 | DL06S05-0308WTG |
Diol-120 | 5 | 120 | 8.0 X 30 | DL12S05-0308WTG |
Diol-200 | 5 | 200 | 8.0 X 30 | DL20S05-0308WTG |
Diol-300 | 5 | 300 | 8.0 X 30 | DL30S05-0308WTG |
Analysis Columns(Glass Columns)
Packing material | Particle size (µm) |
Pore size (Å) |
Column size Inner diameter x length (mm) |
Product Number |
---|---|---|---|---|
Diol-60 | 5 | 60 | 8.0 X 300 | DL06S05-3008FG |
8.0 X 500 | DL06S05-5008FG |
|||
Diol-120 | 5 | 120 | 8.0 X 300 | DL12S05-3008FG |
8.0 X 500 | DL12S05-5008FG | |||
Diol-200 | 5 | 200 | 8.0 X 300 | DL20S05-3008FG |
8.0 X 500 | DL20S05-5008FG |
|||
Diol-300 | 5 | 300 | 8.0 X 300 | DL30S05-3008FG |
8.0 X 500 | DL30S05-5008FG |