YMC-Triart Series

YMC-Triart Series are next-generation organic hybrid silica-based columns that emphasize versatility. The main features are superior durability, good peak shape for all kinds of compounds, and reproducibility. With the same selectivity across different particle sizes, smooth method transfer between UHPLC and HPLC can be performed. Moreover, various bonded phases supplement the performance of the C18 phase, allowing separations that C18 columns cannot achieve. YMC-Triart Prep preparative packing materials with great chemical stability are also available.

Lineup

Columns

  USP
Class No.
Particle size
(μm)
Pore size
(Å)
Carbon content
(%)*
Usable
pH range
100% aqueous
compatibility
Triart C18 L1 1.9
3
5
120 20 1.0 - 12.0
Triart C18 ExRS L1 80 25 ×
Triart C8 L7 120 17 ×
Triart Phenyl L11 120 17 1.0 - 10.0
Triart PFP L43 120 15 1.0 - 8.0
Triart Diol-HILIC L20 120 12 2.0 - 10.0 -

*Containing 8% for hybrid silica base material.

Packing materials

  Particle size
(μm)
Pore size
(Å)
Carbon content
(%)*
Usable
pH range
Triart SIL 3, 5 120 - -
Triart C18 20 1.0 - 12.0
Triart C8 17
Triart Prep SIL 10, 15, 20 120, 200 - -
Triart Prep C18-S 120 20 2.0 - 10.0
Triart Prep C8-S 200 13

*Containing 8% for hybrid silica base material.

Features

Great chemical durability in an expanded pH range provided by hybrid particles

With innovative surface modification on organic hybrid silica, YMC-Triart columns show great chemical durability and can be used over a wide pH range. Even at high-pH or high-temperature conditions, the lifetimes of YMC-Triart C18 and C8 are more than 10 times greater than those of conventional C18 columns and a few times greater than those of commercially available high-alkaline-resistant C18 columns.

Durability at a high pH

pH 11.5, 40ºC

Column 5 µm, 150 X 4.6 mmI.D.
Eluent 50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Flow rate 1.0 mL/min
Temperature 40ºC
Sample benzyl alcohol

Durability at a low pH at a high temperature

pH 1, 70ºC

Test condition

The columns are kept in acetonitrile/water/TFA (10/90/1, pH 1) at 70ºC, and tested for performance every 20 hours under the condition of column inspection below.

Column inspection
Column 5 µm, 50 X 2.0 mmI.D.
Eluent acetonitrile/water (60/40)
Flow rate 0.2 mL/min
Temperature 37ºC
Sample butyl benzoate

Great peak shapes without adsorption/peak tailing

The peak tailing or fronting of ionic compounds is often caused by adsorption to residual silanol groups and/or surface impurities resulting from base materials or manufacturing processes.
YMC-Triart, based on a hybrid silica material with low metal impurities and rigorously endcapped, provides symmetrical peak shapes for all types of compounds.

Basic compounds
Eluent 20 mM KH2PO4-K2HPO4 (pH 6.9) /acetonitrile (65/35)
Detection UV at 235 nm
Sample 1. Chlorpheniramine
2. Dextromethorphan
3. Propyl paraben (I.S.)

Coordination compounds
Eluent acetonitrile/0.1% H3PO4 (40/60)
Detection UV at 254 nm
Sample 1. Hinokitiol
2. Methyl benzoate (I.S.)
Acidic compounds
Eluent 10 mM CH3COOH-CH3COONH4 (pH 4.2) /acetonitrile (75/25)
Detection UV at 254 nm
Sample 1. Salicylic acid
2. Methyl paraben (I.S.)
3. Cinnamic acid

 
Column 150 X 3.0 mmI.D. or 150 X 4.6 mmI.D.
Flow rate 0.425 mL/min for 3.0 mmI.D.,
1.0 mL/min for 4.6 mmI.D.
Temperature 40ºC

Identical selectivity and excellent peak shapes across various particle sizes

YMC-Triart columns have identical selectivity and excellent peak shapes for basic (ionic) compounds across all particle sizes, including 1.9 μm. This allows predictable scale-up from UHPLC to conventional HPLC and even to semi-preparative LC, and vice versa. In contrast, commercially available C18 columns often show some differences in selectivity, retention, and peak shape between different particle sizes.

Basic compounds

  1. Chlorpheniramine
  2. Dextromethorphan
  3. Propyl paraben(I.S.)
Column 50 X 2.0 mmI.D. or 2.1 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.2 mL/min
Temperature 40℃
Detection UV at 235 nm

Comparison of separation selectivity among YMC-Triart columns

A mixture that consists of compounds with various characteristics is analyzed with reversed-phase Triart columns. In addition to hydrophobic interactions, secondary interactions such as π–π interactions and polar interactions differ from column to column. These parameters have a great impact on the retention capacity (k′) and separation factor (α). By utilizing the differences in the separation characteristics, a wide range of compounds can be well separated with Triart columns.

  1. Amitriptyline
  2. 8-Quinolinol
  3. Testosterone
  4. Naphthalene
  5. Ibuprofen
  6. Propylbenzene
  7. n-Butylbenzene
  8. o-Terphenyl
  9. Triphenylene
Column 5 µm, 150 X 3.0 mmI.D.
Eluent 20 mM KH2PO4-H3PO4 (pH 3.1)/methanol (25/75)
Flow rate 0.425 mL/min
Temperature 40℃
Detection UV at 265 nm
Injection 4 µL
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