150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.

YMC-Pack PVA-Sil

YMC-Pack PVA-Sil

YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external and internal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. It is an excellent choice for Supercritical Fluid Chromatography (SFC).

YMC-Pack PVA-Sil

YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external and internal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. It is an excellent choice for Supercritical Fluid Chromatography (SFC).

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L24, polyvinyl alcohol
Stationary phase
USP L24, polyvinyl alcohol
Particle size
5 μm
Particle size
5 μm
Pore size
120 Å
Pore size
120 Å
Usable pH range
2.0 ~ 9.5
Usable pH range
2.0 ~ 9.5
Recommended pH range
2.0 ~ 7.5
Recommended pH range
2.0 ~ 7.5

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

Separation of potato lipids

YMC-Pack PVA-Sil can be used to separate a range of potato lipids.

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)

Column YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate 1 to 2 mL/min
Eluent A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient
Tmin 0 5 15 40 40.1 45 50
B% 0 20 52 52 70 0 0
C% 0 0 4 14 0 0 0
Flow (mL/min) 1 1 1 1.4 1.4 2 2
Nebuliser temperature 25ºC, Evaporation temperature : 35ºC
Detector ELSD
Sample
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
mgDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol

 

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150 Beach Road, #25-02 Gateway West, Singapore 189720.

Tel : +65 6392 2643

Fax : +65 6392 2856

Email : sales@ymc.sg

YMC Singapore Tradelinks Pte. Ltd.

150 Beach Road, #25-02 Gateway West, Singapore 189720.

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