We have 15 different kinds of C18 columns. They are divided in 4 groups, Hybrid silica based YMC-Triart columns, Core-shell type silica based Meteoric Core columns, Silica-based columns and Polymer-based YMC-Pack PolymerC18.
YMC-Triart columns exhibit extraordinary stability, low operating pressure, and excellent performance. Meteoric Core columns provide outstanding resolution compared to fully porous silica based columns.
To break down further within the Silica-based columns, they are divided in 3 groups, Pro series, YMC-Pack series (excl. PolymerC18) and J’sphere series. Pro series features in a superior performance and excellent reproducibility. Pro series with an efficient endcapping technology is superior to YMC-Pack ODS series and J’sphere series. You can find more information in the following C18 column selection guide.
ODS-A and AM are conventional ODS. ODS-AQ provides a lower rate of carbon content and is suitable for separation of hydrophilic compounds. ODS-A and -AM have the same basic physical properties such as the base material, the rate of carbon content and the separation characteristics, however -AM is produced under more strict Standards of Quality Control.
Polyamine II and PA-G are chemically bonded with polyamine where NH2 is with aminopropyl group. Polyamine II and PA-G are superior to NH2 in durability. They also have difference in selectivity. Polyamine II and PA-G have different ligand structure of polyamine. Polyamine II is superior to PA-G in durability.
General ODS (C18) packing material is a silica gel bonded with octadecyl groups. This is in the result of a reaction between silanol groups and octadecyl groups on the silica surface. However some silanol groups remain after the reaction. It is impossible for all the silanol groups to react because of steric hindrance of octadecyl groups. Such residual silanol groups create a secondary interaction in chromatography, which, in many cases, affects on chromatograms by, in general, causing a peak tailing of basic compounds or irreversible absorption to the column. Therefore, a secondary silanization on residual silanol groups with unbulky silanization reagents should be performed. This process is called “endcapping”. Trimethylsilane (TMS) is commonly used in “endcapping” process.
“Triart C18”, “Hydrosphere C18” and “ODS-AQ” columns can be used with 100% aqueous mobile phase. On conventional ODS columns, retention time is shortened due to the incompatibility between water and material surface with high hydrophobicity. Water tends to be expelled from the pores on material. The retention time hardly shortened on “YMC-Triart C18”, “Hydrosphere C18” and “ODS-AQ” because they are capable of solvation between mobile phase and hydrophilic surface by reducing the density of C18 functional groups.
Applicable pH range and temperature
|Column Type||pH range||Usable temperature range|
|Regular use||Upper limit|
|Triart C18, C18 ExRS, C8||1 – 12||20-40℃||pH 1-7 : 90℃|
pH 7-12 : 50℃
|Triart Phenyl||1 – 10||20-40℃||50℃|
|Triart PFP||1 – 8||20-40℃||50℃|
|Triart Bio C18||1 – 12||20-40℃||pH 1-9 : 90℃|
pH 9-12 : 50℃
|Triart Bio C4||1 – 10||20-40℃||pH 1-7 : 90℃|
pH 7-10 : 50℃
|Pro C18, Hydrosphere C18||2 – 8||20-40℃||50℃|
|Pro C18 RS||1 – 10||20-40℃||50℃|
|J’sphere ODS-H80||1 – 9||20-40℃||50℃|
|PolymerC18||2 – 13||25-35℃||65℃|
|Triart Diol-HILIC||2 – 10||20-40℃||50℃|
|Reversed-phase (Other than mentioned above) Normal phase (SIL, Polyamine II)||2 – 7||20-40℃|
*The given data is subject to change depends on product types. Those data should be confirmed with Instruction manual when the column is used.
When columns are not used for a long time, keep them in a cool place after replacing with the shipping solvent as indicated in the attached inspection report. Do not keep the column in the mobile phase with salt or acid regardless of whether or not it is in a short period of time. Close the airtight stopper tightly to prevent the solvent from volatilizing.
Perform an inspection test under the same conditions as the inspection report attached to the column at the time of purchase. Columns are evaluated to be effective and have no change in performance if the result indicates no irregularity in retention time, theoretical plate number, peak asymmetry, etc. Columns which indicate no irregularity in the said criteria after using several years from purchase, however, may have changes in separa