150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.
YMC BioPro HIC BF is a hydrophobic interaction chromatography (HIC) column with butyl (C4) stationary phase bonded to hydrophilic polymer resin. BioPro HIC BF is useful for the separation of low hydrophobic proteins as well as purifying proteins such as antibody without denaturation.
YMC BioPro HIC BF is a hydrophobic interaction chromatography (HIC) column with butyl (C4) stationary phase bonded to hydrophilic polymer resin. BioPro HIC BF is useful for the separation of low hydrophobic proteins as well as purifying proteins such as antibody without denaturation.
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
BioPro HIC BF shows stronger retention of proteins due to higher hydrophobicity of its stationary phase compared to other commercially available HIC columns. This indicates that BioPro HIC BF could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 25ºC |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) 2. Trastuzumab (0.5 mg/mL) |
The papain-digests of NISTmAb samples with and without the oxidization were analyzed by using BioPro HIC BF column. The Fab and Fc fragments were characterized from the chromatogram of the papain-digested MAb. In the chromatogram of the papain-digested oxidized MAb, the multiple peaks appeared at earlier elution times compared to the peaks assigned to the Fab and Fc fragments, and would be corresponding to the oxidized fragments. According to the previous report*, the oxidized fragments elute earlier than the non-oxidized ones.
*Journal of Chromatography A, 2008, 1214, 81-89
| Column | BioPro HIC BF 4 µm, 100 X 4.6 mmI.D. |
| Eluent | A) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) containing 2.0 M (NH4)2SO4 B) 100 mM NaH2PO4-Na2HPO4Â (pH 7.0) 40-80%B (0-10 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 5 µL (0.5 mg/mL) |
150 Beach Road, #25-02 Gateway West, Singapore 189720.
Tel : +65 6392 2643
Fax : +65 6392 2856
Email : sales@ymc.sg
150 Beach Road, #25-02 Gateway West, Singapore 189720.
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