BioPro HIC HT

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BioPro HIC HT

YMC BioPro HIC HT is a hydrophobic interaction chromatography (HIC) column with butyl (C4) stationary phase bonded to hydrophilic polymer resin. YMC BioPro HIC HT is suitable for analysis of biopharmaceuticals such as antibody-drug conjugates (ADC’s) and drug-to-antibody ratio (DAR).

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Column care and use instruction download link

BioPro HIC HT

Catalogue Download Link

Column care and use instruction download link

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Ideal for rapid analysis of ADC with high resolution

BioPro HIC HT has high pressure tolerance with excellent chromatographic resolution by optimized packing condition. This enables rapid analysis without losing the resolution at high pressure, which is too high for other commercially available HIC column.

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

DAR analysis by using BioPro HIC HT and competitor columns under two mobile phase conditions varying 2-propanol content was carried out. The competitor A column having high hydrophobicity did not elute peak #5 completely with the mobile phase containing less than 15% 2-propanol. The competitor B column eluted all peaks with the 10% 2-propanol mobile phase condition, but it showed poor separation between peaks #4 and #5. On the other hand, BioPro HIC HT eluted all peaks completely with good separation and another peak was partially separated on the peak #3. Additionally, it is noteworthy that the same DAR values were observed at both mobile phases.

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Specification:

Base

Hydrophilic polymer

Base

Hydrophilic polymer

Stationary phase

Butyl (C4)

Stationary phase

Butyl (C4)

Particle size

2.3 μm

Particle size

2.3 μm

Pore size

Non-porous

Pore size

Non-porous

Usable pH range

2 ~ 12

Usable pH range

2 ~ 12

Temp. range

10-60°C

Temp. range

10-60°C

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Ideal for rapid analysis of ADC with high resolution

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (85/15)
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Column	100 X 4.6 mmI.D.
Eluent	A) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 1.0 M (NH₄)₂SO₄ B) 20 mM NaH₂PO₄-Na₂HPO₄ (pH 7.0)/2-propanol (90/10) or (85/15) 0-100%B (0-15 min), 100%B (15-20 min), 0%B (20-35 min)
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Brentuximab vedotin (2.5 mg/mL)

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Please contact us for product with other sizes.

Particle size (µm)	Column size Inner diameter X length (mm)	Product number
2.3	4.6 X 33	BHH00SQ3-H346PTH
2.3	4.6 X 100	BHH00SQ3-1046PTH

Columns

BioPro HIC HT

BioPro HIC HT

BioPro HIC HT

Specification:

Specification:

Specification:

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Specification:

Specification:

Specification:

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Specification:

Specification:

Specification:

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

Ideal for rapid analysis of ADC with high resolution

Innovative surface chemistry for drug-to-antibody ratio (DAR) analysis

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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