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Phone : +65 6392 2643
Email : sales@ymc.sg
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150 Beach Road, #25-02 Gateway West, Singapore 189720.
Phone : +65 6392 2643
Email : sales@ymc.sg

BioPro IEX Column

BioPro IEX Column

BioPro IEX Column is made of hydrophilic polymer beads with low nonspecific adsorption. BioPro IEX Columns are for separation of proteins, peptides, and nucleic acids. Porous (QA and SP columns) type offers excellent binding capacity with high recovery while the non-porous type (QF and SF columns) offers high efficiency and exceptional resolution.

Catalogue Download Link
Column care and use instruction download link
Column care and use instruction download link

BioPro IEX Column

BioPro IEX Column is made of hydrophilic polymer beads with low nonspecific adsorption. BioPro IEX Columns are for separation of proteins, peptides, and nucleic acids. Porous (QA and SP columns) type offers excellent binding capacity with high recovery while the non-porous type (QF and SF columns) offers high efficiency and exceptional resolution.

Catalogue Download Link
Column care and use instruction download link

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Specification:

BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK
BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity [reference value]
(meq/mL-resin)		 0.09 0.09 0.09 0.24
Dynamic binding capacity [reference value]
(mg/mL-resin)		 >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. 35%B (70 mM NaCl）
Gradient slope 0.25%B/min (0.5 mM NaCl)
Flow rate 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature 30°C
Detection UV at 280 nm
Injection 10 μL
Sample Humanized monoclonal IgG1 (1 mg/mL)

 

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)		 Eluted amount
(mg/mL-resin)		 Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

 

Breakthrough curve:

Column BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity 3.0 cm/min
Sample 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

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  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.

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