150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.
BioPro IEX Column is made of hydrophilic polymer beads with low nonspecific adsorption. BioPro IEX Columns are for separation of proteins, peptides, and nucleic acids. Porous (QA and SP columns) type offers excellent binding capacity with high recovery while the non-porous type (QF and SF columns) offers high efficiency and exceptional resolution.
BioPro IEX Column is made of hydrophilic polymer beads with low nonspecific adsorption. BioPro IEX Columns are for separation of proteins, peptides, and nucleic acids. Porous (QA and SP columns) type offers excellent binding capacity with high recovery while the non-porous type (QF and SF columns) offers high efficiency and exceptional resolution.
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity [reference value] (meq/mL-resin) |
0.09 | 0.09 | 0.09 | 0.24 |
| Dynamic binding capacity [reference value] (mg/mL-resin) |
>110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4 - 60 ℃ | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity [reference value] (meq/mL-resin) |
0.09 | 0.09 | 0.09 | 0.24 |
| Dynamic binding capacity [reference value] (mg/mL-resin) |
>110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4 - 60 ℃ | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity [reference value] (meq/mL-resin) |
0.09 | 0.09 | 0.09 | 0.24 |
| Dynamic binding capacity [reference value] (mg/mL-resin) |
>110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4 - 60 ℃ | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
|---|---|---|---|---|
| Matrix | hydrophilic porous polymer beads | hydrophilic non-porous polymer beads | ||
| Particle size (µm) | 5 | 3, 5 | ||
| Charged group | -CH2N+(CH3)3 | -CH2CH2CH2SO3- | -CH2N+(CH3)3 | -CH2CH2CH2SO3- |
| Counter ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange capacity [reference value] (meq/mL-resin) |
0.09 | 0.09 | 0.09 | 0.24 |
| Dynamic binding capacity [reference value] (mg/mL-resin) |
>110 (BSA) | >70 (human-IgG) | >12 (BSA) | >10 (human-IgG) |
| Usable temperature | 4 - 60 ℃ | |||
| Usable pH range | 2-12 | |||
| Column material | PEEK | |||
The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.
| Eluent | A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl |
| Initial gradient conc. | 35%B (70 mM NaCl） |
| Gradient slope | 0.25%B/min (0.5 mM NaCl) |
| Flow rate | 180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.) |
| Temperature | 30°C |
| Detection | UV at 280 nm |
| Injection | 10 μL |
| Sample | Humanized monoclonal IgG1 (1 mg/mL) |
Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.
Comparisons in dynamic binding capacity (DBC) and recovery for BSA
| Dynamic binding capacity (mg/mL-resin, 10% breakthrough) |
Eluted amount (mg/mL-resin) |
Recovery* (%) |
|
|---|---|---|---|
| BioPro IEX QA | 126 | 120 | 95 |
| Brand T (porous Q type) | 73 | 58 | 79 |
| Brand G (porous Q type) | 100 | 35 | 35 |
*Recovery: (Eluted amount/Dynamic binding capacity) X 100
Breakthrough curve:
| Column | BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D. |
| Linear velocity | 3.0 cm/min |
| Sample | 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer |
| Equilibration buffer | 20 mM Tris-HCl (pH 8.6) |
| Elution buffer | 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl |
| Detection | UV at 280 nm |
The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.
| Eluent | A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl |
| Initial gradient conc. | 35%B (70 mM NaCl） |
| Gradient slope | 0.25%B/min (0.5 mM NaCl) |
| Flow rate | 180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.) |
| Temperature | 30°C |
| Detection | UV at 280 nm |
| Injection | 10 μL |
| Sample | Humanized monoclonal IgG1 (1 mg/mL) |
Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.
Comparisons in dynamic binding capacity (DBC) and recovery for BSA
| Dynamic binding capacity (mg/mL-resin, 10% breakthrough) |
Eluted amount (mg/mL-resin) |
Recovery* (%) |
|
|---|---|---|---|
| BioPro IEX QA | 126 | 120 | 95 |
| Brand T (porous Q type) | 73 | 58 | 79 |
| Brand G (porous Q type) | 100 | 35 | 35 |
*Recovery: (Eluted amount/Dynamic binding capacity) X 100
Breakthrough curve:
| Column | BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D. |
| Linear velocity | 3.0 cm/min |
| Sample | 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer |
| Equilibration buffer | 20 mM Tris-HCl (pH 8.6) |
| Elution buffer | 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl |
| Detection | UV at 280 nm |
*Recovery: (Eluted amount/Dynamic binding capacity) X 100
The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.
| Eluent | A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl |
| Initial gradient conc. | 35%B (70 mM NaCl） |
| Gradient slope | 0.25%B/min (0.5 mM NaCl) |
| Flow rate | 180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.) |
| Temperature | 30°C |
| Detection | UV at 280 nm |
| Injection | 10 μL |
| Sample | Humanized monoclonal IgG1 (1 mg/mL) |
Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA