BioPro IEX Column

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BioPro IEX Column

BioPro IEX Column is made of hydrophilic polymer beads with low nonspecific adsorption. BioPro IEX Columns are for separation of proteins, peptides, and nucleic acids. Porous (QA and SP columns) type offers excellent binding capacity with high recovery while the non-porous type (QF and SF columns) offers high efficiency and exceptional resolution.

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BioPro IEX Column

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Column care and use instruction download link

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Higher resolution and higher efficiency compared to competitor's column

The separation of MAb is compared between YMC BioPro IEX SF column and competitor’s WCX column under the same gradient conditions at pH 5.6. BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Specification:

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

	BioPro IEX QA	BioPro IEX SP	BioPro IEX QF	BioPro IEX SF
Matrix	hydrophilic porous polymer beads		hydrophilic non-porous polymer beads
Particle size (µm)	5		3, 5
Charged group	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-	-CH₂N⁺(CH₃)₃	-CH₂CH₂CH₂SO₃^-
Counter ion	Cl^-	Na⁺	Cl^-	Na⁺
Ion-exchange capacity [reference value] (meq/mL-resin)	0.09	0.09	0.09	0.24
Dynamic binding capacity [reference value] (mg/mL-resin)	>110 (BSA)	>70 (human-IgG)	>12 (BSA)	>10 (human-IgG)
Usable temperature	4 - 60 ℃
Usable pH range	2-12
Column material	PEEK

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Higher resolution and higher efficiency compared to competitor's column

Eluent	A) 20 mM MES-NaOH (pH 5.6) B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc.	35%B (70 mM NaCl）
Gradient slope	0.25%B/min (0.5 mM NaCl)
Flow rate	180 cm/hr (0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature	30°C
Detection	UV at 280 nm
Injection	10 μL
Sample	Humanized monoclonal IgG1 (1 mg/mL)

High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA exhibits superior dynamic binding capacity (DBC) and recovery due to its low nonspecific adsorption.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

	Dynamic binding capacity (mg/mL-resin, 10% breakthrough)	Eluted amount (mg/mL-resin)	Recovery* (%)
BioPro IEX QA	126	120	95
Brand T (porous Q type)	73	58	79
Brand G (porous Q type)	100	35	35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curve:

Column	BioPro IEX QA 50 X 4.6 mmI.D. Brand T (porous Q Type) 50 X 4.6 mmI.D. Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity	3.0 cm/min
Sample	1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer	20 mM Tris-HCl (pH 8.6)
Elution buffer	20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection	UV at 280 nm

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

Packing material	Particle size (µm)	Pore size	Column size inner diameter x length (mm)	Product number
BioPro IEX QA	5	Porous	4.6 X 30	QAA0S05-0346WP
			4.6 X 50	QAA0S05-0546WP
			4.6 X 100	QAA0S05-1046WP
BioPro IEX SP	5	Porous	4.6 X 30	SPA0S05-0346WP
			4.6 X 50	SPA0S05-0546WP
			4.6 X 100	SPA0S05-1046WP
BioPro IEX QF	3	Non-porous	4.6 X 30	QF00S03-0346WP
			4.6 X 50	QF00S03-0546WP
			4.6 X 100	QF00S03-1046WP
	5	Non-porous	4.6 X 30	QF00S05-0346WP
			4.6 X 50	QF00S05-0546WP
			4.6 X 100	QF00S05-1046WP
BioPro IEX SF	3	Non-porous	4.6 X 30	SF00S03-0346WP
			4.6 X 50	SF00S03-0546WP
			4.6 X 100	SF00S03-1046WP
	5	Non-porous	4.6 X 30	SF00S05-0346WP
			4.6 X 50	SF00S05-0546WP
			4.6 X 100	SF00S05-1046WP

BioPro IEX Column

BioPro IEX Column

BioPro IEX Column

Specification:

Specification:

Specification:

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Specification:

Specification:

Specification:

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Specification:

Specification:

Specification:

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

Higher resolution and higher efficiency compared to competitor's column

High binding capacity and high recovery of biomolecules

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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