150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.
This column is made of ultrapure silica with minimal metal content and very low concentrations of residual silanols. This makes it an ideal phase for the analysis of basic pharmaceuticals and chelating compounds even under LC-MS conditions. Hydrosphere C18 can be used to separate polar compounds under highly aqueous mobile phase conditions.
This column is made of ultrapure silica with minimal metal content and very low concentrations of residual silanols. This makes it an ideal phase for the analysis of basic pharmaceuticals and chelating compounds even under LC-MS conditions. Hydrosphere C18 can be used to separate polar compounds under highly aqueous mobile phase conditions.
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
Retention time of nucletic acid bases is compared before and after one night storage in 100% water. Hydrosphere C18 provides stable retention and remarkable reproducibility compared to conventional ODS columns which have declining retention on the next day. Hydrosphere C18 is suitable for separation of polar compounds under highly aqueous mobile phase.
After the operation, the column was stored overnight (about 15 hours)
| Column size | 150 X 4.6 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) |
| Flow Rate | 1.0 mL/min |
| Temperature | 37ºC |
| Detection | UV at 254 nm |
Hydrosphere C18 with 2 µm particle size shows similar column efficiency with 60% less in column pressure compared to other sub-2 µm columns used in UHPLC. Hence it can be used for ultra fast analysis with conventional LC system.
| Column | 50 X 2.0 or 2.1 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Temperature | 25ºC |
| Sample | Butyl benzoate |
150 Beach Road, #25-02 Gateway West, Singapore 189720.
Tel : +65 6392 2643
Fax : +65 6392 2856
Email : sales@ymc.sg
150 Beach Road, #25-02 Gateway West, Singapore 189720.
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