150 Beach Road, #25-02 Gateway West, Singapore 189720.
Phone : +65 6392 2643
Email : sales@ymc.sg
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150 Beach Road, #25-02 Gateway West, Singapore 189720.
Phone : +65 6392 2643
Email : sales@ymc.sg

YMC-Triart Bio C4

YMC-Triart Bio C4

This organic/inorganic hybrid silica based column with butyl functional group is a wide pore colume suitable for separation of proteins and oligonucleotides as well as separation of intact antibodies under high temperature. Its excellent durability makes analysis up to 90°C at pH 1-7, and 50°C at pH 7-10 possible. It is also compatible with LC-MS.

Catalogue Download Link
Column care and use instruction download link
Column care and use instruction download link

YMC-Triart Bio C4

This organic/inorganic hybrid silica based column with butyl functional group is a wide pore colume suitable for separation of proteins and oligonucleotides as well as separation of intact antibodies under high temperature. Its excellent durability makes analysis up to 90°C at pH 1-7, and 50°C at pH 7-10 possible. It is also compatible with LC-MS.

Catalogue Download Link
Column care and use instruction download link

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Specification:

Base
Organic/inorganic hybrid silica
Base
Organic/inorganic hybrid silica
Stationary phase
USP L26, C4
Stationary phase
USP L26, C4
Particle size
1.9 µm, 3 µm, 5 µm
Particle size
1.9 µm, 3 µm, 5 µm
Pore size
300 Å
Pore size
300 Å
Usable pH range
1 ~10
Usable pH range
1 ~10
Endcapping
Yes
Endcapping
Yes

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

Useful for separation of intact antibodies under high temperature

In the analysis of intact antibodies from 40°C up to 90°C, good peak shape was obtained at 70°C and onwards. Its excellent durability enables analysis of antibodies even at high temperature up to 90°C.

Column YMC-Triart Bio C4 (3 µm, 300 Å)
150 X 3.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
30-60%B (0-15 min), 90%B (15-30 min)
Flow rate 0.4 mL/min
Detection UV at 220 nm
Sample Humanized monoclonal IgG1
Injection 4 µL

 

Good peak shape with mobile phase suitable for LC-MS

Good peak shape was obtained using YMC-Triart Bio C4 compared to competitor C4. As YMC-Triart Bio C4 is compatible with mobile phase containing formic acid, it is suitable for LC-MS analysis.

  1. Cytochrome c (Horse heart)
  2. Insulin (Bovine pancreas)
  3. Transferrin (Human)
  4. BSA
  5. B-Lactogloblin A (Bovine)
  6. a-Chymotrypsinogen A (Bovine pancreas)
  7. Ovalbumin
Column size 150 X 3.0 mmI.D or 4.6 mml.D.
Eluent  A) water/formic acid (100/0.1)
B) acetonitrile/formid acid (100/0.1)
10-95%B (0-15 min)
Flow rate 0.4 mL/min for 3.0 mml.D.
1.0 mL/min for 4.6 mml.D.
Temperature 40℃
Detection UV at 220 nm

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YMC Singapore Tradelinks Pte. Ltd.

  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

  • Technical Support
  • Download
  • Selection Guide
  • Application Note
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Company Info

Product Category

YMC Singapore Tradelinks Pte. Ltd.

  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.

Newsletter

  • Subscribe now and get the interesting information from us.

© 2022 YMC. All rights reserved.

Designed by Wondertabs

  • Technical Support
  • Download
  • Selection Guide
  • Application Note
Facebook Twitter Youtube

Company Info

Product Category

YMC Singapore Tradelinks Pte. Ltd.

  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.

Newsletter

  • Subscribe now and get the interesting information from us.

© 2022 YMC. All rights reserved.

Designed by Wondertabs

Newsletter

  • Subscribe now and get the interesting information from us.
Facebook Twitter Youtube

Company Info

Product Category

Support

YMC Singapore Tradelinks Pte. Ltd.

  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.
© 2022 YMC. All rights reserved. | Designed by Wondertabs
  • 150 Beach Road, #25-02 Gateway West, Singapore 189720.

Newsletter

  • Subscribe now and get the interesting information from us.
Facebook Twitter Youtube

Company Info

Columns
System
Packing Material
Flow Reactor
Contract Purification Service
© 2022 YMC. All rights reserved. | Designed by Wondertabs

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150 Beach Road, #25-02 Gateway West, Singapore 189720.

Tel : +65 6392 2643

Fax : +65 6392 2856

Email : sales@ymc.sg

YMC Singapore Tradelinks Pte. Ltd.

150 Beach Road, #25-02 Gateway West, Singapore 189720.

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