YMC-Pack Diol

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YMC-Pack Diol

YMC-Pack Diol is a size exclusion chromatography column packed with dihydroxypropyl-bonded silica. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Diol-60 is suitable for separation of peptides or oligosaccharides whose molecular weights are below 10,000.

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Column care and use instruction download link

YMC-Pack Diol

Catalogue Download Link

Column care and use instruction download link

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Stationary phase

USP L20, L33, L59, dihydroxypropyl

Particle size

2μm, 3 μm, 5 μm

Particle size

2μm, 3 μm, 5 μm

Pore size

60Å, 120Å, 200Å, 300Å

Pore size

60Å, 120Å, 200Å, 300Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

<10,000 ~ 1,000,000

Molecular weight

<10,000 ~ 1,000,000

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

Lineup

Column	Pore size (Å)	Particle size (µm)	Usable pH range	Molecular weight range
Diol-60	60	3, 5	5.0-7.5	below 10,000
Diol-120	120	3, 5		1,000-100,000
Diol-200	200	2, 3, 5		5,000-300,000
Diol-300	300	2, 3, 5		20,000-1,000,000

Calibration curves of various proteins

lgM ( 900,000 MW)
Thyroglobulin (670,000 MW)
lgA ( 390,000 MW)
Fibrinogen (340,000 MW)
y-Globulin (158,000 MW)
lgG (150,000 MW)
Transferrin (75,000 MW)
HSA (human serum albumin) (66,000 MW)
a1 - Antitrypsin (50,000 MW)
Ovalbumin (45,000 MW)
Carbonic anhydrase (30,000 MW)
Trypsin inhibitor (20,100 MW)
Myoglobin (17,000 MW)
a- Lactalbumin (14,100 MW)
Ribonuclease A (13,700 MW)
Cytochrome c (12,400 MW)

Pore Size (5 m)

Column	YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.5 mL/min
Temperature	25°C
Detection	UV at 280 nm

Particle Sizes (Diol-300)

Column	YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

Glutamate dehydrogenase ( 290,000 MW)
Lactate dehydrogenase ( 142,000 MW)
Enolase ( 67,000 MW )
Adenylate kinase ( 32,000 MW )
Cytochrome c ( 12,400 MW )

Column	YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.7 mL/min
Temperature	ambient
Detection	UV at 280 nm

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

UHPLC Columns (Max. pressure : 45 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-200	2	200	4.6 X 150	DL20S02-1546PTH
Diol-200	2	200	4.6 X 300	DL20S02-3046PTH
Diol-300	2	300	4.6 X 150	DL30S02-1546PTH
Diol-300	2	300	4.6 X 300	DL30S02-3046PTH

Analysis Columns (Stainless Columns, Max. pressure : 20 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	3	60	4.6 X 300	DL06S03-3046WT
	5	60	4.6 X 300	DL06S05-3046WT
			8.0 X 300	DL06S05-3008WT
			8.0 X 500	DL06S05-5008WT
Diol-120	3	120	4.6 X 300	DL12S03-3046WT
	5	120	4.6 X 300	DL12S05-3046WT
			8.0 X 300	DL12S05-3008WT
			8.0 X 500	DL12S05-5008WT
Diol-200	3	200	4.6 X 300	DL20S03-3046WT
	5	200	4.6 X 300	DL20S05-3046WT
			8.0 X 300	DL20S05-3008WT
			8.0 X 500	DL20S05-5008WT
Diol-300	3	300	4.6 X 300	DL30S03-3046WT
	5	300	4.6 X 300	DL30S05-3046WT
			8.0 X 300	DL30S05-3008WT
			8.0 X 500	DL30S05-5008WT

Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 30	DL06S05-0308WTG
Diol-120	5	120	8.0 X 30	DL12S05-0308WTG
Diol-200	5	200	8.0 X 30	DL20S05-0308WTG
Diol-300	5	300	8.0 X 30	DL30S05-0308WTG

Analysis Columns (Glass Columns, Max. pressure : 5 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	8.0 X 300	DL06S05-3008FG
Diol-60	5	60	8.0 X 500	DL06S05-5008FG
Diol-120	5	120	8.0 X 300	DL12S05-3008FG
Diol-120	5	120	8.0 X 500	DL12S05-5008FG
Diol-200	5	200	8.0 X 300	DL20S05-3008FG
Diol-200	5	200	8.0 X 500	DL20S05-5008FG
Diol-300	5	300	8.0 X 300	DL30S05-3008FG
Diol-300	5	300	8.0 X 500	DL30S05-5008FG

Semi-prep Columns (Stainless Columns, Max. pressure : 10 MPa)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 300	DL06S05-3020WT
Diol-60	5	60	20 X 500	DL06S05-5020WT
Diol-120	5	120	20 X 300	DL12S05-3020WT
Diol-120	5	120	20 X 500	DL12S05-5020WT
Diol-200	5	200	20 X 300	DL20S05-3020WT
Diol-200	5	200	20 X 500	DL20S05-5020WT
Diol-300	5	300	20 X 300	DL30S05-3020WT
Diol-300	5	300	20 X 500	DL30S05-5020WT

Semi-prep Guard Columns (Stainless Columns)

Packing material	Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
Diol-60	5	60	20 X 50	DL06S05-0520WTG
Diol-120	5	120	20 X 50	DL12S05-0520WTG
Diol-200	5	200	20 X 50	DL20S05-0520WTG
Diol-300	5	300	20 X 50	DL30S05-0520WTG

YMC-Pack Diol

YMC-Pack Diol

YMC-Pack Diol

Specification:

Specification:

Specification:

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Specification:

Specification:

Specification:

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Specification:

Specification:

Specification:

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

Lineup

Calibration curves of various proteins

Separation of standard protein markers

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Company Info

Product Category

YMC Singapore Tradelinks Pte. Ltd.

Newsletter

© 2022 YMC. All rights reserved.

Designed by Wondertabs

Company Info

Product Category

YMC Singapore Tradelinks Pte. Ltd.

Newsletter

© 2022 YMC. All rights reserved.

Designed by Wondertabs

Company Info

Product Category

YMC Singapore Tradelinks Pte. Ltd.

Newsletter

© 2022 YMC. All rights reserved.

Designed by Wondertabs

Newsletter

Company Info