150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.

YMC-Pack Diol

YMC-Pack Diol

YMC-Pack Diol is a size exclusion chromatography column packed with dihydroxypropyl-bonded silica. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Diol-60 is suitable for separation of peptides or oligosaccharides whose molecular weights are below 10,000.

YMC-Pack Diol

YMC-Pack Diol is a size exclusion chromatography column packed with dihydroxypropyl-bonded silica. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Diol-60 is suitable for separation of peptides or oligosaccharides whose molecular weights are below 10,000.

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Specification:

Base
Silica
Base
Silica
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Stationary phase
USP L20, L33, L59, dihydroxypropyl
Particle size
2μm, 3 μm, 5 μm
Particle size
2μm, 3 μm, 5 μm
Pore size
60Å, 120Å, 200Å, 300Å
Pore size
60Å, 120Å, 200Å, 300Å
Usable pH range
5 ~ 7.5
Usable pH range
5 ~ 7.5
Molecular weight
<10,000 ~ 1,000,000
Molecular weight
<10,000 ~ 1,000,000

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000

 

Calibration curves of various proteins

YMC-Pack Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 1,000 to 1,000,000. Calibration curves of proteins using YMC-Pack Diol of different particle sizes are similar and this enables easy transfer between UHPLC and HPLC.

 

  1. lgM ( 900,000 MW)
  2. Thyroglobulin (670,000 MW)
  3. lgA ( 390,000 MW)
  4. Fibrinogen (340,000 MW)
  5. y-Globulin (158,000 MW)
  6. lgG (150,000 MW)
  7. Transferrin (75,000 MW)
  8. HSA (human serum albumin) (66,000 MW)
  9. a1 - Antitrypsin (50,000 MW)
  10. Ovalbumin (45,000 MW)
  11. Carbonic anhydrase (30,000 MW)
  12. Trypsin inhibitor (20,100 MW)
  13. Myoglobin (17,000 MW)
  14. a- Lactalbumin (14,100 MW)
  15. Ribonuclease A (13,700 MW)
  16. Cytochrome c (12,400 MW)

 

Pore Size (5 m)

Column YMC-Pack Diol. 300 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Particle Sizes (Diol-300)

Column YMC-Pack Diol. 300 X 4.6 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

 

Separation of standard protein markers

Diol-200 is suitable for separation of proteins with molecular weight between 5,000 to 300,000.

  1. Glutamate dehydrogenase ( 290,000 MW)
  2. Lactate dehydrogenase ( 142,000 MW)
  3. Enolase ( 67,000 MW )
  4. Adenylate kinase ( 32,000 MW )
  5. Cytochrome ( 12,400 MW )
Column YMC-Pack Diol. 500 X 8.0 mml.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm

Related Product

Packing Material

CHIRAL ART Coated type

Packing Material

CHIRAL ART Coated type

Packing Material

CHIRAL ART Coated type

Packing Material

CHIRAL ART Immobilized type

Packing Material

CHIRAL ART Immobilized type

Packing Material

CHIRAL ART Immobilized type

Columns

YMC-Pack PVA-Sil

Columns

YMC-Pack PVA-Sil

Columns

YMC-Pack PVA-Sil

Columns

YMC-Pack PA-G

Columns

YMC-Pack PA-G

Columns

YMC-Pack PA-G

Columns

YMC-Pack NH2

Columns

YMC-Pack NH2

Columns

YMC-Pack NH2

Columns

YMC-Pack Polyamine II

Columns

YMC-Pack Polyamine II

Columns

YMC-Pack Polyamine II

Search Product here

150 Beach Road, #25-02 Gateway West, Singapore 189720.

Tel : +65 6392 2643

Fax : +65 6392 2856

Email : sales@ymc.sg

YMC Singapore Tradelinks Pte. Ltd.

150 Beach Road, #25-02 Gateway West, Singapore 189720.

Subscribe to Our Newsletter

Subscribe now and get the interesting information from us.