YMC-SEC MAB

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YMC-SEC MAB

YMC-SEC MAB is a silica-based size exclusion chromatography column suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs). YMC-SEC MAB exhibits excellent durability and batch-to-batch reproducibility.

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Column care and use instruction download link

YMC-SEC MAB

Catalogue Download Link

Column care and use instruction download link

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

A humanized monoclonal antibody and an ADC were analyzed using YMC-SEC MAB. Excellent peak shape and good separation between aggregates and a monomer peak were observed. YMC-SEC MAB is effective for characterization and quality control of antibody drugs.

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

YMC-SEC MAB shows reproducible separation without any change in theoretical plate number and elution time even after repeated separation of a monoclonal antibody for more than 500 times due to its superior durability.

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Specification:

Base

Silica

Base

Silica

Stationary phase

USP L20, L59, dihydroxypropyl

Stationary phase

USP L20, L59, dihydroxypropyl

Particle size

3 μm

Particle size

3 μm

Pore size

250 Å

Pore size

250 Å

Usable pH range

5 ~ 7.5

Usable pH range

5 ~ 7.5

Molecular weight

10,000 ~ 700,000

Molecular weight

10,000 ~ 700,000

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH₂PO₄-K₂HPO₄ (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH₂PO₄-Na₂HPO₄ (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Please feel free to contact us for other dimensions.

Particle size (µm)	Column size inner diameter X length (mm)	Product number
3	4.6 X 300	DLM25S03-3046WT
3	8.0 X 300	DLM25S03-3008WT

Columns

YMC-SEC MAB

YMC-SEC MAB

YMC-SEC MAB

Specification:

Specification:

Specification:

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Specification:

Specification:

Specification:

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Specification:

Specification:

Specification:

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

Suitable for separation of aggregates of antibodies or antibody drug conjugates (ADCs)

Excellent durability

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YMC Singapore Tradelinks Pte. Ltd.

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YMC Singapore Tradelinks Pte. Ltd.

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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YMC Singapore Tradelinks Pte. Ltd.

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© 2022 YMC. All rights reserved.

Designed by Wondertabs

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