The YMC-Triart stationary phases are packed in PEEK-lined stainless steel column hardware, complete with PEEK frits. It is designed to eliminate peak shape deterioration caused by metal components. It is suitable for analysis of coordination compounds. High pressure tolerance is guaranteed.
The YMC-Triart stationary phases are packed in PEEK-lined stainless steel column hardware, complete with PEEK frits. It is designed to eliminate peak shape deterioration caused by metal components. It is suitable for analysis of coordination compounds. High pressure tolerance is guaranteed.
Metal coordination compounds which have phosphate group in their structure tend to show poor peak shape by interacting with metal, such as stainless steel column. In this nucleotides analysis, better peak shape was achieved with the metal free column. The metal free column is also effective for highly sensitive analysis using LC-MS.
Column | YMC-Triart C18, 3 μm, 50 x 2.1mmI.D. |
Eluent | 5 mM HCOONH4 |
Flow rate | 0.21 mL/min |
Temperature | 25°C |
Detection | UV at 265 nm |
Injection | 1 μL (10 μg/mL) |
Tubing or HPLC system components could induce peak shape deterioration. Nucleotides were analyzed on an ordinary HPLC system and a “non-metal” HPLC system which consists of a