150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.

YMC*GEL/YMC*GEL HG/YMC-Omega

YMC*GEL/YMC*GEL HG/YMC-Omega

YMC*GEL is a high density and high strength silica based packing material with various functional groups. The particle size ranges from 3 μm up to 150 μm for open column chromatography. They are suitable for long term repacking into dynamic axial compression (DAC) column due to their excellent mechanical stability.

https://www.ymc.co.jp/data/download/YMC_GEL_ODS_e.pdf
https://www.ymc.co.jp/data/download/YMC_GEL_C8_C4_HG_e.pdf
https://www.ymc.co.jp/data/download/YMC_GEL_CN_TMS_Ph_HG_e.pdf
https://www.ymc.co.jp/data/download/YMC_GEL_SIL_HG_e.pdf

YMC*GEL/YMC*GEL HG/YMC-Omega

YMC*GEL is a high density and high strength silica based packing material with various functional groups. The particle size ranges from 3 μm up to 150 μm for open column chromatography. They are suitable for long term repacking into dynamic axial compression (DAC) column due to their excellent mechanical stability.

Specification:

Specification:

Specification:

Excellent mechanical stability (YMC*GEL HG)

The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.

Packing conditions
Column size 100 X 50 mmI.D.
Packing pressure 6.5 MPa
Test conditions
Eluent methanol/water(85/15)
Flow rate 50 mL/min

 

Suitable for separation of insulin and for scale-up purification

YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.

Analytical condition (1), (3)
Column YMC-Pack C8 (5 µm, 200 Å)
150 X 4.6 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 0.8 mL/min
Temperature ambient
Detection UV at 220 nm
Preparative condition (2)
Column YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 100 mL/min
Temperature ambient
Detection UV at 220 nm

Excellent mechanical stability (YMC*GEL HG)

The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.

Packing conditions
Column size 100 X 50 mmI.D.
Packing pressure 6.5 MPa
Test conditions
Eluent methanol/water(85/15)
Flow rate 50 mL/min

 

Suitable for separation of insulin and for scale-up purification

YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.

Analytical condition (1), (3)
Column YMC-Pack C8 (5 µm, 200 Å)
150 X 4.6 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 0.8 mL/min
Temperature ambient
Detection UV at 220 nm
Preparative condition (2)
Column YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 100 mL/min
Temperature ambient
Detection UV at 220 nm

Excellent mechanical stability (YMC*GEL HG)

The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.

Packing conditions
Column size 100 X 50 mmI.D.
Packing pressure 6.5 MPa
Test conditions
Eluent methanol/water(85/15)
Flow rate 50 mL/min

 

Suitable for separation of insulin and for scale-up purification

YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.

Analytical condition (1), (3)
Column YMC-Pack C8 (5 µm, 200 Å)
150 X 4.6 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 0.8 mL/min
Temperature ambient
Detection UV at 220 nm
Preparative condition (2)
Column YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 100 mL/min
Temperature ambient
Detection UV at 220 nm