150 Beach Road, #25-02 Gateway West, Singapore 189720.
150 Beach Road, #25-02 Gateway West, Singapore 189720.
YMC*GEL is a high density and high strength silica based packing material with various functional groups. The particle size ranges from 3 μm up to 150 μm for open column chromatography. They are suitable for long term repacking into dynamic axial compression (DAC) column due to their excellent mechanical stability.
YMC*GEL is a high density and high strength silica based packing material with various functional groups. The particle size ranges from 3 μm up to 150 μm for open column chromatography. They are suitable for long term repacking into dynamic axial compression (DAC) column due to their excellent mechanical stability.
The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.
| Packing conditions | |
|---|---|
| Column size | 100 X 50 mmI.D. |
| Packing pressure | 6.5 MPa |
| Test conditions | |
| Eluent | methanol/water（85/15） |
| Flow rate | 50 mL/min |
YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.
| Analytical condition (1), (3) | |
|---|---|
| Column | YMC-Pack C8 (5 µm, 200 Å) 150 X 4.6 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 0.8 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Preparative condition (2) | |
|---|---|
| Column | YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 100 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.
| Packing conditions | |
|---|---|
| Column size | 100 X 50 mmI.D. |
| Packing pressure | 6.5 MPa |
| Test conditions | |
| Eluent | methanol/water（85/15） |
| Flow rate | 50 mL/min |
YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.
| Analytical condition (1), (3) | |
|---|---|
| Column | YMC-Pack C8 (5 µm, 200 Å) 150 X 4.6 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 0.8 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Preparative condition (2) | |
|---|---|
| Column | YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 100 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
The pressure between YMC*GEL HG in DAC column and competitor's gel was compared for more than 10 rounds of repacking. Only the pressure of YMC*GEL HG remains constant after 10 repacking cycles due to its high mechanical stability. The SEM images also show that YMC*GEL HG remains relatively intact after 13th repacking.
| Packing conditions | |
|---|---|
| Column size | 100 X 50 mmI.D. |
| Packing pressure | 6.5 MPa |
| Test conditions | |
| Eluent | methanol/water（85/15） |
| Flow rate | 50 mL/min |
YMC*GEL and YMC*GEL HG with wide pore are suitable for separation of peptides, such as insulin and proteins. With the similar performance between analytical packing material (YMC*GEL) and preparative packing material (YMC*GEL HG), the scale-up is easy.
| Analytical condition (1), (3) | |
|---|---|
| Column | YMC-Pack C8 (5 µm, 200 Å) 150 X 4.6 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 0.8 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Preparative condition (2) | |
|---|---|
| Column | YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D. |
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 100 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |